Fig. 9
From: Crosslinked-hybrid nanoparticle embedded in thermogel for sustained co-delivery to inner ear
FL and HK protect from cisplatin ototoxicity in zebrafish. A-L: Representative micrographs of neuromast hair cells immunostained for the hair cell marker, otoferlin (red). D-F: FL alone at 33µM, 17µM, or 2µM concentrations. G-I: HK alone at 33µM, 17µM, or 2µM concentrations. J-L: FL and HK in combination at 17mM, 8.5mM, or 3mM concentrations (each). NP = empty cHy-NPs. Scale bar: 10 μm. M: quantification of the number of hair cells per neuromast. Results are expressed as a percentage of protection, with 100% representing control animals and 0% cisplatin-treated fish. A maximum of three neuromasts were inspected per fish were inspected in 10–12 fish. Statistical analysis: One-way ANOVA followed by Dunnett post-test for multiple comparisons. ***p < 0.01 compared to cisplatin alone. #p < 0.05 compared to FL + HK17. ns = not significant.