Fig. 5
From: Radiation-induced ferroptosis via liposomal delivery of 7-Dehydrocholesterol
Biodistribution and safety of N-7DHC-lipos. (a) Scheme of the biodistribution experiments, which were performed in H1299 tumor-bearing nude mice. 7DHC-lipos, labeled with DiR, and conjugated with NTSmut (w/ NTSmut) or without NTSmut (w/o NTSmut) were administered i.v. Whole-body fluorescence imaging was performed 0.5, 4, and 24 h after nanoparticle injection (n = 3 mice). (b) Representative whole-body fluorescence images. (c) Tumor-to-muscle fluorescence ratios, based on ROI analysis of imaging results from (b). (d) Uptake of 7DHC-lipos, w/ and w/o coupling with NTSmut, based on ROI analysis of ex vivo imaging results with dissected organs, which was normalized to signals in the muscle. (e) Fluorescence microscopy of tumor tissues taken from both w/ NTSmut and w/o NTSmut groups. Blue, DAPI; red, 7DHC-lipos. Scale bar, 50 μm. (f) Scheme of the toxicity study. N-7DHC-lipos in PBS (10 mg/kg) or PBS alone were injected i.v. to healthy BALB/C mice (n = 3 mice). The animals were euthanized after two weeks. Blood and major organs were collected for CBC, biochemistry, and histopathology. (g) ALT and BUN results. (h) Selected CBC results. RBC, red blood cells; Hct, hematocrit test; WBC, white blood cells; Hgb, hemoglobin; Lym, lymphocytes; Mon, monocytes; Eos, eosinophils; Bas, basophils. (i) H&E staining of major organ tissues. Scale bar, 200 μm. Data are presented as mean ± SD. Statistical difference was evaluated using two-tailed Student’s t-test in (c-d and g-h). **p < 0.01, ***p < 0.001; ns, p > 0.05