Fig. 6

Therapeutic efficacy of N-7DHC-lipos, which was evaluated in both H1299 tumor-bearing nude mice and LLC1 tumor-bearing C57BL/6 mice. (a) Scheme of efficacy studies conducted in H1299 tumor-bearing nude mice. 7-DHC-lipos (10 mg/kg) were injected i.v., followed by 5 Gy tumor irradiation at 24 h. A total of three treatments were administered every two days (n = 5 mice). For comparison, PBS alone, 7-DHC-lipos alone, and IR alone were tested. (b) Average tumor growth curves. (c) Animal survival curves. (d) Individual tumor growth curves. (e) Average animal body weight curves. (f) H&E and Ki67 staining of tumor tissue samples. Scale bars, 200 μm. (g) Immunofluorescence microscopy of tumor tissue stained for 4-HNE (n = 3 mice). H1299 tumor-bearing mice were treated with a dose of 7-DHC-lipos plus IR, and tumor samples were collected after euthanasia 24 h after IR. Scale bar, 50 μm. (h) Scheme of efficacy studies performed in LLC1 tumor-bearing C57BL/6 mice. The same nanoparticle and irradiation dose were used as in the H1299 study (n = 5 mice). (i) Average tumor growth curves. (j) Animal survival curves. (k) Average animal body weight curves. (l) H&E and Ki67 staining of tumor tissue samples from different treatment groups. Scale bars, 200 μm. Data are presented as mean ± SD. Statistical difference was evaluated using one-way ANOVA for tumor volume in (b, i), and Mantel-Cox test for survival in (c, j). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001