Fig. 6
From: Nanoplastics-mediated physiologic and genomic responses in pathogenic Escherichia coli O157:H7
Transcriptomic analysis of the E. coli O157:H7 in planktonic (PL) and biofilm (BF) states in the presence of NPs. a Heatmap of the expression of genes in different sample groups. Red and green indicate up- and down-regulation, respectively. b Number of differentially expressed genes (DEGs) in different NP-treatment groups with respect to the controls (not exposed to NPs). c Principal component analysis (PCA) of different sample groups. Clustering of biological replicates from the same sample group indicates a greater similarity between replicates. d–f Volcano plots of DEGs in planktonic cells exposed to NP(−), NP( +), and NP(0) compared against the PL_control (mid-log phase cells). g, h Volcano plots of DEGs in biofilm cells (15-day) exposed to NP(−) and NP( +), and compared against BF_control (biofilm not exposed to NPs). Note. Details of key differentially expressed genes are listed in Table S1 of the supplementary file