Fig. 5

M2-sEVs increased NSC proliferation and neuronal differentiation after OGD. (A) A diagram showing the experimental design for investigating the uptake of M2-sEVs by NSC in vitro. M2-sEVs or PBS were stained with DiI dye, and then ultracentrifugated to harvest M2-sEVs, and then M2-sEVs-DiI was cocultured with NSC, finally the cells was imaged under microscope. (B) Representative immunofluorescence images of DAPI and DiI in NSC. Scale bar, 50 μm. (C) A diagram showing the experimental design for exploring the proliferation and differentiation of NSC after OGD. (D) Representative WB bands and analysis of MAP-2 and GFAP proteins in NSC among different groups. n = 5 per group. (E) Representative immunofluorescence images of EdU and Nestin in NSC in the indicated groups. Scale bar, 50 μm. (F) Representative immunofluorescence images of GFAP and MAP-2 in NSC in the indicated groups. Scale bar, 50 μm. (G) Quantification of EdU⁺ proliferated cells and percentage of EdU⁺Nestin⁺ proliferated NSC in DAPI + cells. n = 5 per group. (H) Determining the percentage of MAP-2⁺ cells or GFAP⁺ cells in DAPI⁺ cells. n = 5 per group