Fig. 8

M2-sEVs transfer miR93-5p and miR25-3p to decrease the expression of TGFBR, PTEN, and FOXO3 in NSC. (A) A scheme of the experimental design. M2-sEVs or PBS were administered via tail veil injection following MCAO. The expressin of proteins in SVZ was determined by WB. (B-C) Representative WB bands (B) and quantification of protein level (C) of TGFBR, PTEN, and FOXO3 in NSC 14 days following MCAO. n = 4 per group. (D) A demonstration of the experimental design. M2-sEVs or PBS was added into the culture medium of NSC after OGD, followed by measurement of the proteins levels in NSC by WB. (E-F) Representative WB bands (E) and quantification of protein level (F) of TGFBR, PTEN, FOXO3 in NSC after OGD. n = 4 per group. (G) The design of the experiment. Briefly, After OGD, NSC cocultured with PBS, M2-sEV with or without miRNA inhibitor (M2-sEVs + IN or M2-sEVs + NC). (H-I) Representative WB bands (H) and the corresponding quantification of protein levels (I) of TGFBR, PTEN, and FOXO3 in NSC (n = 4 per group)