Fig. 5
From: Piezoelectric-immunomodulatory electrospun membrane for enhanced repair of refractory wounds
In vitro antibacterial activity of PLLA@Ga + US. (a) The sensitivity to alkaline phosphatase activity. (b) The ONPG hydrolysis activity. (c-d) Leakage of cell contents after exposure to various treatments was investigated by monitoring the absorbance of extracellular material at 260 nm (A260) and 280 nm (A280) corresponding to nucleic acids and proteins, respectively. (e-f) Morphology of bacteria from different treatment groups as observed by TEM. (g) Assessment of ROS generation was conducted using DCFH-DA fluorescent probes by FCM. (h) Assessment of ROS generation was conducted using DCFH-DA fluorescent probes by microplate reader. (i) Volcano map of differentially expressed genes (DEGs). (j-k) KEGG pathway enrichment analysis. Data are presented as mean ± SD (n = 3). “ns” represent “no significant”; *P < 0.05; **P < 0.01; ***P < 0.001