Fig. 5
ETN@Fe7S8 promotes cell migration and tube formation. (A) IF staining of CD31 for the wounds on days 3 and 7, and (B) the quantitative analysis. (C) Images of tube formation with different treatments, and (D) quantitative analysis. (E) Images of HUVECs migration with different treatments for 0 and 24 h, and (F) quantitative analysis. (G) Images of tube formation with different treatments, and (H) quantitative analysis. (I) The cell proliferation rate of HUVEC cells on 0, 24, 48 h. (J) Western blot analysis of VEGFA protein levels in HUVEC cells. (K) Western blot analysis of AKT, P-AKT, PI3K, and P-PI3K protein levels in HUVEC cells*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001