Fig. 7

In vitro evaluationof ATRA-NLC on IFN-γ-treated DPCs. (A) Cell viability of different formulations on DPCs. (B) Cell cycle analyzed by flow cytometer. (C, D) Quantification of cell cycle phases. (E) The effect of ATRA-NLC on the activity of alkaline phosphatase (ALP) in the DPCs. Scale bar: 20 μm. (F) The expression levels of Ki-67, β-catenin, and CD200 were detected by Western blot. Statistical analysis of the expression levels of CD200 (G), Ki-67 (H), β-catenin (I). n = 3, ^*p < 0.05, ^**p < 0.01, vs model; ^#p < 0.05, ^##p < 0.01, vs ATRA cream