Fig. 3

Cellular uptake, cytotoxicity, and in vitro NO, ROS, and ONOO^- generation. (A) CLSM images of LLC cells, RAW264.7 cells and MLE-12 cells after incubation with D-RhB or D-RhB@M. Scale bar = 20 μm. (B) CLSM images of LLC cells, RAW264.7 cells and MLE-12 cells after incubation with DID@Lipo, DID@M, and blocked DID@M. Scale bar = 50 μm. (C) Relative viability of LLC cells after different treatments (n = 3). (D) Relative viability of LLC cells treated with 50 µM DHA-N@M + 6 Gy X-ray in the presence of 25 µM Nec-1, 50 µM Z-VAD-Fmk, or 1 µM Fer-1 (n = 3). * and *** denote p < 0.05 and p < 0.001, respectively. CLSM images of (E) NO, (F) ROS, and (G) ONOO^- in LLC cells after different treatments. Scale bar = 20 μm